Hairy Root Induction of Taxus baccata L. by Natural Transformation with Rhizobium rhizogenes

نویسندگان

چکیده

Paclitaxel (Taxol®) is a potent anticancer agent, but the widespread pharmaceutical use of paclitaxel hampered by its limited availability due to low accumulation levels in native yew (Taxus spp.) plants. Currently, hairy root culture an emerging biotechnological tool that presents several advantages such as reduced costs and higher specialized metabolite production, therefore, application production can be commercial medicinal interest. The objective present study was induce Taxus baccata L. transformation with wild type Rhizobium rhizogenes A4 strain. Thus, T. inoculated three different inoculation methods: (a) ex vitro seedlings direct injection liquid bacterial culture; (b) needles co-culturing bacteria; (c) shoots dipping culture. Hairy roots were formed only from method, efficiency 14.3%. Formation observed two months after inoculation. This project forms basis for establishment cultures paclitaxel.

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ژورنال

عنوان ژورنال: Horticulturae

سال: 2022

ISSN: ['2311-7524']

DOI: https://doi.org/10.3390/horticulturae9010004